|
|
Date: 2022
Authors:
MEGAN E. BROWN, Smithsonian Conservation Biology Institute
KIM BOARDMAN, International Crane Foundation
KATIE L. EDWARDS, North of England Zoological Society
|
DOWNLOAD
|
|
|
Citation:
Brown, M. E., K. Boardman, and K. L. Edwards. 2022. Initial evaluation of cyclic adenosine monophosphate enzyme immunoassay for use with crane semen samples. Proceedings of the North American Crane Workshop 15:150-154.
Abstract:
The management of ex situ and in situ populations of many wildlife species requires detailed knowledge of a species’
reproductive biology. For species such as cranes, where artificial insemination is a critical component of ex situ management
strategies, understanding normal sperm function is especially important. Previous research has shown that captive cranes
exhibit highly variable production and quality of semen samples produced by individual males and high levels of variation of
cell concentration and motility across different species. Cyclic adenosine monophosphate (cyclic AMP) has been implicated
in regulating sperm function, such as cell motility, and may affect an individual’s ability to successfully fertilize. Here we
demonstrate the feasibility of an enzyme-linked immunosorbent assay (ELISA) for measuring cyclic AMP produced by crane
sperm to facilitate future research into its role in sperm function and fertilization.
The North American Crane Working Group provides free and open access to articles in Proceedings of the North American Crane Workshop. No additional permission is required for unrestricted use, distribution, or reproduction in any medium, provided that the original work here is properly cited. Complete Proceedings are also available for download or purchase.
© 2022 North American Crane Working Group
| |
